From 46504cf0609eadefc7009f1eff55a26328a984b9 Mon Sep 17 00:00:00 2001
From: Bernardo Aguzzoli Heberle
 <81333350+bernardo-heberle@users.noreply.github.com>
Date: Fri, 22 Mar 2024 07:30:34 -0400
Subject: [PATCH] Update README.md

---
 README.md | 5 ++++-
 1 file changed, 4 insertions(+), 1 deletion(-)

diff --git a/README.md b/README.md
index 8aa1d66..3fd20b7 100755
--- a/README.md
+++ b/README.md
@@ -97,7 +97,10 @@ Many of the parameters for this step are based on dorado basecaller, see their [
 ## Parameters for step 2 (Alignment Filtering and Quality Control):
 
 ```
---step
+--step                                  <Type: Integer. Options are integers 1, 2, 3. Select 1 for basecalling. Select 2 for alignment
+                                        filtering and quality control. Select 3 for methylation call pre-processing
+                                        with modkit and generating a thorough multiQC report for sequencing stats.
+                                        This parameter needs to be set for the pipeline to run. Default: "None">
 
 --steps_2_and_3_input_directory          <This parameter must be set to the output path of step 1 set with the out_dir parameter.
                                           For example "./results/<out_dir>". Default = "None">
-- 
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