diff --git a/README.md b/README.md
index e166f3470c547b4f00958a9fc069f4e18809e49c..954d8bec93f921d70f5fc3e3f79a0b8b39c84d6b 100755
--- a/README.md
+++ b/README.md
@@ -30,6 +30,8 @@ git clone https://github.com/bernardo-heberle/DCNL_NANOPORE_PIPELINE
 
 ### 5) Set NXF_SINGULARITY_CACHEDIR environment variable to your desired directory:
 
+#### Substitute `/<your>/<desired>/<path>/` in the codeblock below for the path to the directory you would like to store your singularity images in. Make sure the directory exists before executing the pipeline.
+
 ```
 echo "" >> ~/.bash_profile && echo 'NXF_SINGULARITY_CACHEDIR="/<your>/<desired>/<path>/"' >> ~/.bash_profile && echo 'export NXF_SINGULARITY_CACHEDIR' >> ~/.bash_profile && . ~/.bash_profile
 ```